The present study aimed to investigate the elementary and advanced teachers’ formative assessment literacy of speaking skill in Teaching Persian to Non-Persian Speakers context. To this end, 26 teachers of teaching Persian to non-Persian speakers, mostly female, took part in this study. To collect data about their speaking assessment practices, they were primarily involved in a semi-structured interview related to the components of aims, contents, and methods of their speaking assessment. Afterward, they answered five scenarios. The interviews were based on Xu and Brown’s (2016) framework, and the scenarios were based on Chappius and Stiggins’ (2009) assessment quality and standards. In this phase, in addition to aims, content, and methods, and two additional components of giving feedback and students’ participation in assessing the speaking skill were examined. The interview results in relation to  the aims for the instructors of elementary and advanced levels shed light on six criteria including comprehension, learning and progress, teaching method and curriculum efficacy, weak and strong points, communicative and practical competence, self-confidence and feedback; in relation to content, grammatical accuracy, pronunciation, fluency, interaction and communication, pragmatics, content, turn taking and participation, and spoken and written differences were recognized; and in relation to  methods, class conversation, audio and video files, modeling and memorizing, game and competition, summary telling, question and response, playing roles, giving speech, problem solving and authentic activities were identified. Quantitative analysis of the interviews and scenarios indicated statistical differences among the two groups of elementary and advanced teachers.